From September 8, 2023, to September 16, 2023, a business trip was undertaken by Dr. Sc., Professor Savitskaya I.S. and Ph.D., Associate Professor Kistaubaeva A.S. to the Ecole Nationale Superieure d'Agronomie et des Industries Alimentaires, Nancy, France.

The purpose of the business trip was as follows: to conduct collaborative scientific research as outlined in the project schedule of AR09259491, titled "Biotechnology Utilizing Polysaccharide Matrix with Probiotic Biofilms for the Production of Combined Dairy Products.

The results obtained from the collaborative research would be incorporated into a publication in a journal with an impact factor of at least 80 percentile. Metagenomic studies of intestinal flora were conducted.

Metagenomic analysis of intestinal microflora was carried out at the Department of Biotechnology, Al-Farabi Kazakh National University.

The following methods were employed: DNA was extracted from fecal samples of volunteers aged 24 to 35 years who had been taking the dietary supplement "Lactocel" for 15 days. The samples were initially collected using Zymo Research Quick-DNA kits and then processed using Microprep kits (Catalog No: D6012) and ZymoBiomics DNA Microprep kits (Catalog No: D4301) following the manufacturer's instructions. The concentration of fecal DNA was measured using a Nanodrop 2000/2000c spectrophotometer (ThermoFisher), and a genotypic method based on the analysis of nucleotide sequence similarity of the 16S rRNA gene was also conducted. DNA extraction and purification from bacterial cells were performed using a specialized kit provided by the Ecole Nationale Superieure d'Agronomie et des Industries Alimentaires.

The quality of genomic DNA was assessed through agarose gel electrophoresis, and the DNA quantity was determined using a Nano Drop ND 1000 spectrophotometer. Gene fragments were obtained through polymerase chain reaction (PCR) and amplified using sets of oligonucleotide primer pairs. Nucleotide sequence determination was carried out using an automated genetic analyzer ABI 3730xl (Applied Biosystems, USA). The sequencing results were analyzed using computer software programs, including Vector NTI: ContigExpress and AlignX. Multiple sequence alignments were conducted using the ClustalX program.