Good Health and Well-Being
The Scientists of the Department conduct scientific research, which also covers the goals of sustainable development
Theme of the project (supervisor, implementation timeline) | Brief description of the project |
Goal, objectives of the project |
Expected/obtained results |
Development novel nanobodies for efficient neutralization and as specific and sensitive probe for rapid detection rabies virus (Bissenbaev А.К., 2022-2024 гг.) |
Rabies is deadliest viral infection with nearly 100% fatality. There is currently no effective treatment. The problem of rabies in Kazakhstan remains unresolved, natural foci of the disease are constantly recorded, which requires an increase in the effectiveness of rabies prevention and control measures. The only treatment for rabies is the use of specific prophylaxis with vaccines and anti-rabies immunoglobulin (RIG). The RIG is derived from the plasma either of horses or humans. Both products have several limitations relate to safety, supply, and cost.
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Project goal: Development of novel nanobodies for the efficient neutralization of RABV. Engineering highly specific and sensitive nanobody-derived probe for rapid detection RABV in the immunoassays.
Objectives of the project: 1. expression of RABV-G protein as correctly folded recombinant protein and evaluation of antigenicity of the expression product. 2. Construction of a cDNA-VHH library by phage display methods and selection of RABV-G protein specific nanobodies. 3. Study of RABV neutralization efficacy of mono-, bi- and trivalent RABV specific nanobodies on mice challenged by a lethal dose of RABV.
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Results: Human rabies poses a serious threat to the health of the population of Kazakhstan, natural foci of the disease are constantly recorded, which requires an increase in the effectiveness of rabies prevention and control measures. This project is aimed at creating new nanobodies to effectively neutralize the rabies virus, which has great potential. In this project, specific nanoantibodies against the rabies virus were isolated from the blood serum of camels using reverse transcription and PCR methods. Various forms of nanoantibodies have been created that contribute to the effective neutralization of the rabies virus. The recombinant glycoprotein (G-protein) of the rabies virus was cloned and expressed in bacterial and yeast systems. A specific nanobody with alkaline phosphatase has been created, which makes it possible to accelerate the process of detection of the rabies virus in samples by enzyme immunoassay using a single step. Thus, the development of specific nanobodies against the rabies virus can lead to a significant reduction in the cost of existing vaccines, solve the problem of supplying remote areas, and has great prospects for further commercial production.
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Study of the mismatch-specific thymine-DNA-glycosylase-initiated aberrant pathway for excisional repair of complex DNA damage in vitro and in vivo (Taipakova S.M., 2022-2024) | In this project, using biochemical and genetic approaches, we aim to decipher and characterize at the molecular level the mechanisms involved in the aberrant BER pathway initiated by DNA glycosylases. In addition, identification of critical DNA damages that can cause aberrant repair in cells will provide a mechanistic understanding of the environmental and genetic factors associated with aging and degenerative diseases, and therefore help develop new prevention and therapeutic strategies. |
Project goal: The main goal of the project is to decipher and characterize at the molecular level the mechanisms involved in the aberrant BER pathway initiated by the mismatch-specific thymine-DNA glycosylase in vitro and in vivo, and to evaluate the physiological role of aberrant BER in the accumulation of spontaneous and damage-induced DNA mutations in living cells.
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Results: |