Sturgeon aquaculture production has increased enormously in the last years due to the high demand for caviar on the global market. However, due to the widespread and often uncontrolled use of antibiotics, number of antibiotic-resistant bacteria has increased dramatically and represent a leading cause of morbidity and mortality. Bacteria species, most commonly.

Project goal: Investigation of the therapeutic potential of parental and chimeric endolysins against the Gram-negative and antibiotic resistant bacterium P. fluorescens, P. putida, A. hydrophila, A. salmonicida and A. sobria.

Objectives of the project:

1. Isolation and physiological, biochemical, molecular identification of P. putida, P. fluorescens, A. hydrophila, A. salmonicida and A. sobria bacterial pathogens from infected sturgeon fish.

2. Construction of a chimeric endolysins with extended lytic activity against bacterial pathogens causing of sturgeon fish diseases in aquaculture.

3. Characterization in vitro and in vivo antibacterial activity of parental and constructed novel chimeric endolysins.

Results:

The development of sturgeon aquaculture activities is accompanied by outbreaks of diseases caused by antibiotic-resistant bacteria, which lead to high mortality.

In this regard, in this project, samples of pathogenic bacteria were isolated from external lesions and internal organs of individual diseased sturgeons (Acipenser baerii) collected at the Educational and Scientific Center for Experimental and Industrial Aquaculture Production LLP (Uralsk, Kazakhstan). Using sequencing of full-length 16S rRNA genes and the gyrase beta subunit gene (gyrB) and phylogenetic analysis of the relationship between isolates with representative species of Pseudomonas and Aeromonas in GenBank, we identified the isolated strains as A. salmonicida, A. bestiarum, A. hydrophila, A. veronii , P. parafulva and P. protegens. In isolates of A. hydrophila and A. salmonicida, seven out of ten pathogenicity-associated virulence genes were identified. Antibiotic sensitivity testing showed that absolutely all strains are resistant to the antibiotic groups penicillins, cephalosporins, lincomycins and rifamycins.

Chimeric endolysins with increased lytic activity against bacterial pathogens causing diseases of sturgeons in sturgeon aquaculture have been created. The positive effect of a new antibacterial drug in the treatment of bacterial diseases of sturgeons caused by gram-negative and antibiotic-resistant bacteria is shown.

Project goal: In the present project we are planning isolation and molecular characterization of the wheat putative genes encoding components of TORC1 signaling pathway and their possible role in regulation of wheat seed germination. Knowledge of role this complex network in regulation of plant growth is essential for breeding and biotechnological purposes.

Objectives of the project:

1. Isolation and molecular characterization of the Triticum aestivum putative genes encoding components of TORC1 signalling pathway, i.e., TOR, Raptor, and LST8 and their expression analysis.

2. Characterization of the plant TOR signalling complex by defining its components and perform the functional studies of the pTOR complex.

3. Study of role TaTOR signaling in hormone dependent regulation of protein translation at the early stage of wheat seed germination.

Results:

In the present project, for the first time, were isolated and the molecular characteristics of the wheat putative genes encoding components of TORC1 signaling pathway and their possible role in regulation of wheat seed germination were determined. As a result of the study, the putative TOR, Raptor, and LST8 genes of wheat Triticum aestivum, encoding components of the TORC1 signaling pathway, were isolated and characterized. The expression of these genes in various organs of wheat was studied using real-time RT-PCR and immunoblotting methods, with the obtained antibodies against TaTOR, TaRAPTOR and TaLST8. In vitro and in vivo analyzes of the interaction of recombinant GST-TOR-HEAT-repeat with His-TaRaptor, GST-TaS6K1 with His-TaRaptor were obtained. The effect of GA and TOR inhibitors on ribosomal DNA transcription, on GA-induced protein synthesis in vivo, and the effect of GA and ABA on polysome profiling during the transition from seed to seedling, including aleurone cells, was studied, and mRNA associated with polysomes was identified using the following sequencing methods. generations.